AtlasAlign

AtlasAlign is an innovative open-source plugin designed for researchers working with mouse brain microscopy. Built for Fiji and ImageJ2, it facilitates precise alignment of brain images to standard atlases, enabling detailed review and annotation of regions of interest (ROIs). The latest beta.3 version introduces advanced features such as multichannel and composite viewing, registration that remains fixed to specific slices or time points, and the ability to export selected planes, crops, and masks directly in the original image coordinates. Researchers can refine anatomical alignments, edit ROIs, and review images in a streamlined manner, all while keeping source images read-only to preserve data integrity. The tool supports both individual and batch review workflows, making it ideal for large-scale neuroanatomical studies or quality control in microscopy labs. Its open-source nature and integration with familiar platforms like Fiji make it accessible and customizable for neuroscience labs seeking precise, efficient brain image analysis.